Flow cytometry in hematopathology

The hematopathology department also operates specialized laboratories for molecular diagnostics, flow cytometry, cytogenetic and fish analysis and specialized bone marrow testing. Flow cytometry fc is an immunophenotyping technique in which suspensions of living cells are stained with specific, fluorescently labeled antibodies and then. Wendy shallenberger supervisor of special hematology clinical flow cytometry laboratory phone. While this results in potential diagnostic pitfalls, the tools available for hematopathology, including extensive panels of immunostains, prcbased clonality assessment, and flow cytometry often. The hematopathology bone marrow, lymph nodes, spleen and other sites. The flow cytometry lab is also part of the abramson cancer center and cell sorting shared resource as a core facility at the university of pennsylvania. We offer 10color flow cytometry on peripheral blood, bone marrow, fluid and tissue samples. Fellow rotations include the hematopathology consultation service, the bone marrow and flow cytometry service, molecular pathology and cytogenetics, coagulation and red blood cell special studies, and elective time which may be devoted to additional clinical experience or to research. Specialized areas of hematology are the bone marrow lab, flow cytometry, coagulation lab, and an oncology satellite lab. Flow cytometry hematopathology stanford university school of. Hematopathology leukemialymphoma flow cytometry, blood.

For weekend or holiday emergent cases, please call the laboratory at 3149777864 for recorded instructions to contact the oncall hematopathologist. The society for hematopathology events at the uscap meeting. Flow cytometry and hematopathology clinical laboratories. The flow laboratory offers a full range of diagnostic tests for evaluating hematolymphoid disorders. James verbsky, md, phd describes the uses of flow cytometry for the diagnosis of primary immunodeficiency. The hematopathology division provides specialized diagnostic consultations in malignant and nonmalignant disorders of blood, bone marrow, and lymphoid organs.

The clinical hematology laboratory includes hematology, coagulation, urinalysis, and flow cytometry. We offer worldclass consultative interpretation of the morphology of blood, bone marrow, lymph node and other hematologic specimens, and have specialty expertise in. Hematopathology flow cytometry laboratory schwitalla hall, room 461 3545 vista east of grand st. Flow cytometry general overview flow cytometric immunophenotyping fci is a useful tool in diagnostic hematopathology. Hematopathology clinical flow cytometry laboratory test. All clinical services and programs are part of university of utah health. Diagnostic hematopathology has become an increasingly complex subspecialty, particularly with neoplastic disorders of blood and bone marrow. The hematopathology division provides morphologic and flow cytometric evaluation of bone marrow, lymphomatissues, peripheral blood, and body fluids for the diagnosis of hematopoietic diseases. Flow cytometry of bcell neoplasms pdf download immunophenotypes of chronic myelomonocytic leukemia cmml subtypes by flow cytometry pdf download the specificity of immunophenotypic alterations in blasts in nonacute myeloid disorders pdf download. The albany medical center hematopathology fellowship program is a oneyear program directed toward subspecialty board certification.

A noted authority in clinical flow cytometry and hematopathology, he served for more than ten years as a hematopathologist and director of hematopathology with genzyme genetics, inc. Numerous surface markers, as well as, intracellular markers are offered in combination intended to optimally define leukemic or lymphoid populations. Clinical cytometry focuses on the development and applications of cellular system analysis and array based technologies as applied to clinical practice and translational research. Flow cytometry and hematopathology services pathology. The clinical flow cytometry laboratory utilizes multiparameter 10color instruments and detailed cluster analysis. Common flow cytometry pitfalls in diagnostic hematopathology. We offer 10color flow cytometry on peripheral blood, bone marrow. Hematopathology clinical flow cytometry laboratory. A visual approach to data analysis and interpretation current clinical pathology. Flow cytometry university of pennsylvania pathology and. Jan 10, 2017 dr sumeet gujral from tata memorial hospital, mumbai, india presents a live webinar on application of flow cytometry in diagnosis of acute leukemias.

Flow cytometry fc is an immunophenotyping technique in which suspensions of living cells are stained with specific, fluorescently labeled antibodies and then analyzed with a flow cytometer. Division of hematology, hematopathology laboratory offers testing in diagnosis of leukemias, lymphomas, and other hematopoietic or immunological disorders. This division provides specialized expertise in the evaluation of hematopoietic tissues and blood components. The laboratory is part of our hematopathology division, which includes four fellowshiptrained, boardcertified hematopathologists and seven nyslicensed technologists. Flow cytometry hematopathology stanford university. Applications of flow cytometry and immunohistochemistry to. Ameripaths extensive network of boardcertified hematopathologists and geneticists provides you with the industrys most comprehensive and integrated hematopathology services. While morphologic assessment of blood smears, bone marrow smears, and tissue sections remains the cornerstone of lymphoma and leukemia diagnosis and classification, immunophenotyping is a very valuable and important complementary tool. In hematopathology practice, these cell suspensions are derived from blood, bone marrow, body fluids, or fresh solid tissue samples. Nguyen, 9781588298553, available at book depository with free delivery worldwide. A visual approach to data analysis and interpretation, three physicians highly experienced in laboratory hematopathology and fcm offer a unique systematic approach to fcm data analysis and interpretation based on the visual inspection of dual parameter fcm graphics. For further information, call the laboratory at 3149777864.

The six subspecialty laboratories of the division of hematopathology are. Swerdlow, md director, division of hematopathology telephone. Ganesh raghu, associate professor in pulmonary and critical care medicine, will issue a written report and interpretation for each case. Flow cytometry immunophenotyping of hematopoietic disorders is a complex and demanding exercise that requires a good understanding of cell lineages, developmental pathways, and physiological changes, as well as broad experience in hematopathology. Slucare hematopathologists offer a broad range of diagnostic services, with specialized techniques available through the extensive laboratory resources of. Flow cytometric principles in hematopathology sciencedirect. As part of quest diagnostics, our laboratories routinely employ stateoftheart technologies, including advanced flow cytometry, cytogenetics, fluorescence in situ hybridization fish, polymerase chain reaction. The labe is accredited with cap and meets all clia requirements. Hematopathology flow cytometry department of pathology. Hematopathology team meet the team of experts who specialize in hematopathology. This journal also covers relevant techniques such as flow cytometry, image cytometry, beadbased and slidebased array analyses, as well as other.

Several standard immunophenotyping panels are available for evaluation of suspected leukemia or lymphoma. Flow cytometry hematopathology stanford university school. Hematopathology laboratory medicine and pathology mayo clinic. Flow cytometry in hematopathology a visual approach to data. The clinical flow cytometry laboratory utilizes multiparameter 10color instruments and detailed cluster. Sep 12, 2017 james verbsky, md, phd describes the uses of flow cytometry for the diagnosis of primary immunodeficiency. Stepbystep methods for flow cytometry data analysis and interpretation.

Hematopathology services university of pennsylvania. Flow cytometry in hematopathology a visual approach to. The usc department of pathology offers a oneyear acgme accredited fellowship program in hematopathology, with comprehensive training in morphologic interpretation of bone marrow and lymph node specimens, laboratory hematology, flow cytometric immunophenotyping, immunohistochemistry, molecular hematopathology, special coagulation testing and. Diagnostic hematopathology depends on the applications of flow cytometric immunophenotyping and immunohistochemical. Clinical flow cytometry laboratory evaluations for the workup of possible leukemias or lymphomas can be performed on fresh tissue biopsies ranging from whole lymph nodes to tissue cores from fine needle aspirates, bone marrow aspirates or if necessary biopsies, peripheral blood or body fluids. Types of specimens suitable for fci include peripheral blood, bone marrow bm aspirates, and core biopsies,1. The lab is dedicated to providing all penn investigators access to highquality, costeffective flow cytometric services, and the scientific expertise necessary to use this technology in their. Department of hematopathology md anderson cancer center. Practical flow cytometry in hematopathology a casebased approach. Hematopathology fellowship department of pathology. The division of hematopathology encompasses the sections of hematopathology at the hospital of the university of pennsylvania hup and at the childrens hospital of philadelphia chop and is an integral component in new treatments of hematological malignancies using cellular therapeutic approaches.

Flow cytometry is an ancillary test that aides in the diagnosis of leukemia and lymphoma. The flow cytometry laboratory performs rapid identification and quantification of subsets of cells using laseractivated fluorescent antibodies to cell surface markers. Flow cytometry and hematopathology the ur medicine flow cytometry and hematopathology laboratory offers the regions widest variety of flow cytometry assays. Antibody selection is typically based on one of these standard panels, but is then tailored to the patients individual needs. Reference ranges provided on this web site are for guidance only, and may not reflect the most recent changes. Flow cytometry numerous surface markers, as well as, intracellular markers are offered in combination intended to optimally define leukemic or lymphoid populations. The ur medicine flow cytometry and hematopathology laboratory offers the regions widest variety of flow cytometry assays. More than 20 physicians and nearly 150 laboratory professionals perform the full range of testing approaches, from traditional microscopy to molecular and flow cytometric analyses.